Pat DanielsAssistant Professor of Science
B.S. University of Missouri-Kansas City
M.S. University of Missouri-Kansas City
D.D.S. University of Missouri-Kansas City
Ph.D. University of Missouri-Kansas City
Research Interests and Expertise
I have the academic background to teach the following subjects: anatomy, physiology, biochemistry, histology, cell biology, molecular biology, microbiology, immunology, and introductory biology. Previous research interests include the study of structure-function relationships of specific protein kinases, the study of protein-DNA interactions, and the study of autoimmune diseases. I have substantial experience in various experimental approaches and techniques used in molecular and cellular biology and biochemistry, based primarily on over seven years of postdoctoral research experience. In particular, I have expertise in chromatin immunoprecipitation (see Daniels and Andrews, 2003 and Jiang et al, 2003 for examples of my experiments using this technique).
Daniels, P.J. and G.K. Andrews. 2001. Mechanisms of metal induction of Metallothionein gene expression, in Comprehensive Toxicology, Vol 14, J.P. Vanden Heuvel, G.H. Perdew, W.B. Mattes and W.F. Greenlee, eds., Elsevier Science, New York, pp. 187-200.
Boulatnikov, I.G., Peters, J.L., Nadeau, O.W., Sage, J.M., Daniels, P.J., Kumar, P., Walsh, D.A. and Carlson, G.M. 2009. Expressed Phosphorylase b Kinase and Its αγδ Subcomplex as Regulatory Models for the Rabbit Skeletal Muscle Holoenzyme. Biochemistry, 48,10183-10191.
Boulatnikov, I.G., Nadeau, O.W., Daniels, P.J., Sage, J.M., Jeyasingham, M.D., Villar, M.T., Artigues, A. and Carlson, G.M..2008. The Regulatory β Subunit of Phosphorylase Kinase Interacts with Glyceraldehyde-3-phosphate Dehydrogenase Biochemistry, 48, 7228-7236.
Daniels, P. J. and Andrews, G.K. 2003. Dynamics of the metal-dependent transcription factor complex in vivo at the mouse metallothionein-I promoter. Nucleic Acids Research 31:6710-6721.
Jiang, H., Daniels, P.J. and Andrews, G.K. 2003. Putative zinc-sensing zinc fingers of metal response element-binding transcription factor-1 stabilize a metal-dependent chromatin complex on the endogenous metallothionein-I promoter. J. Biol. Chem 278:30394-30402.
Daniels, P.J., Smirnova, I.V. Bittel, D., Winge, D.R. and Andrews, G.K.. 2002. Mammalian metal response element-binding transcription factor-1 functions as a zinc-sensor in yeast, but not as a sensor of cadmium or oxidative stress. Nucleic Acids Research, 30:3130-3140.
Langmade, S.J., Ravindra, R. Daniels, P.J. and Andrews, G.K. 2000. The transcription factor MTF-1 mediates metal regulation of the mouse ZnT1 gene. J. Biol. Chem. 275:34803-34809.
Daniels, P.J., Gustafson, S.A., French, D., Wang, Y., DePond, W. and McArthur, C.P. 2000. Interferon-mediated block in the cell cycle and altered integrin expression in a differentiated salivary gland epithelial cell line (HSG) grown on Matrigel. Journal of Interferon and Cytokine Research 20:1101-1109.
Daniels, P.J., McArthur, C.P., Heruth, D.P., Rothberg, P.G., Pasztor, L. and Wang, Y. 1999. Cytokine-mediated stimulation of laminin expression and cell growth arrest in a human submandibular gland duct cell line (HSG), Archives of Oral Biology 44:603-615.
McArthur, C.P., Daniels, P.J., Tishk, M. and Mayberry, W.E. 1997. Dental professionals’ role in diagnosing Sjögren’s syndrome, General Dentistry, 45:62-65,.
McArthur, C.P., Daniels, P.J., Kragel, P., Howard, P.F. and Julian, L. 1997. Sjögren’s syndrome salivary gland immunopathology: increased laminin expression precedes lymphocytic infiltration, Journal of Autoimmunity, 10:59-65.